Slides can be roughly divided into two categories: ordinary slides and anti-detachment slides:
✓ Ordinary slides can be used for routine HE staining, cytopathology preparations, etc.
✓ Anti-detachment slides are used for experiments such as immunohistochemistry or in situ hybridization
The main difference between the two is that there is a special substance on the surface of the anti-detachment slide that makes the tissue and the slide adhere more firmly.
The size of glass slides commonly used in microscopes is 76 mm × 26 mm × 1 mm. If the surface of the purchased glass slide has arcs or small protrusions, large air bubbles often appear in the section after sealing, and if the surface cleanliness is not enough, it will also cause problems. The tissue is dissected, or the observation effect is not ideal.
Coverslips are thin, flat glass sheets, commonly square, round, and rectangular, that are placed over a sample viewed under a microscope. The thickness of the cover glass plays a very important role in the imaging effect. I don’t know if you have observed Zeiss objective lenses. Each objective lens has several important parameters, including the requirements for the thickness of the cover glass. .
1. 0.17 in the figure represents that when using this objective lens, the thickness of the cover glass is required to be 0.17mm
2. The representative with the “0″ sign does not need a cover glass
3. If there is a sign “-”, it means that there is no cover glass.
In confocal observation or high magnification observation, the most common one is “0.17″, which means that we need to pay attention to the thickness of the coverslip when we buy coverslips. There are also objectives with correction rings that can be adjusted according to the thickness of the coverslip.
The common types of coverslips on the market are:
✓ #1: 0.13 – 0.15mm
✓ #1.5: 0.16 – 0.19mm
✓ #1.5H: 0.17 ± 0.005mm
Post time: Sep-23-2022