The correct use method of cover glass? What does it do and how does it work?

Microscope is an observation instrument widely used in teaching, scientific research and other aspects. When using a microscope, there is a small “accessory” that Bibuke lacks, that is, the cover glass. Then how should we use the cover glass correctly?

Cover glass should be cleaned before use. Can be washed with clean water, and then gently wipe with gauze or other soft cloth, when making temporary loading, the correct operation of “cover” this step is to gently pick up the cover glass with tweezers, with 45° Angle tilt slowly cover, so that one side of the first contact with the drop on the slide, and then slowly put flat. The purpose of this is to prevent bubbles from appearing under the cover glass. Be sure not to let air in between the observation object and the cover glass. This will affect the observation.

So after we use cover glass, also should be cleaned in time clean and wipe dry in order to prepare for next use, cover glass can be reused actually, although cheap, and is not actually one-time, at ordinary times the word that uses in the home often washes clean essence to rinse ok

If is a friend of demand is higher, can use ultrasonic washing machine cleaning, without ultrasonic washing machine, and very clean, then normal cleaning procedures after washing, and then put a night in chromic acid lotion, and then rinse with distilled water, that is very practical and convenient and effective method.

Figure is small, the function, the main purpose of the cover glass is made by observing the formation of film form, pervious to light, easy to observe, to keep the liquid sample thickness uniform flat layer, the goal is to better focus on in order to make high resolution microscopy, convenient for using capillarity, make add all kinds of reagents (stain, acid and salt solution, etc.) when the formation of gradient. At the same time, the cover glass can also play a role in keeping the observation specimen fixed and flat pressure, and protect the sample from dust and accidental contact. At the same time, it also avoids contaminating the objective lens by accidentally touching the specimen when the objective lens falls. In oil-immersed or water-immersed microscopes, the lid slides to prevent contact between the immersion solution and the sample.

The cover glass can be affixed to the slide block to seal the sample and delay the dehydration and oxidation of the sample. Microbial and cell cultures can be grown directly on the cover glass before being placed on the slide, and samples can be permanently mounted on the slide rather than the slide.

Cover slides come in a variety of widths, lengths and thicknesses. They are usually sized to fit within the boundary of the microscope slide, usually 25 x 75mm in size. Square and round cover slides are generally 20 mm wide or smaller. Rectangular sliders measuring up to 24 x 60mm are available for purchase.

Cover slides are widely available in several standard thicknesses, identified by numbers:

No. 0-0.05 to 0.13 mm thick

N * o *. * 1-1.13 to 0.16 mm thick

N * o *. * 1.5-0.16 to 0.19 mm thick

N * o *. * 1.5 H – 0.17 to 0.18 mm thick

No.2-0.19 to 0.23 mm thick

No. 3-0.25 to 0.35 mm thick

No. 4-0.43 to 0.64 mm thick

The thickness of the cover glass is critical for high resolution microscopes. A typical biological microscope objective is designed for a size 1.5 cover glass slide (0.17 mm thick) with a mounting mount for securing the glass cover to the slide. Using cover slides that deviate from this expected thickness will result in spherical aberration and a reduction in resolution and image intensity. Specialized targets may be used for imaging specimens without cover glasses, or may have correction rings that allow the user to adapt to alternate cover glass thickness.

Cover glass plays an important role in the process of using a microscope. Do you know the above points and points to pay attention to?

Post time: Apr-26-2022